Haley Lab, University of Oregon

Pt-based Coordination Complexes

Partner site with the DeRose lab

This collaboration successfully combines the synthetic expertise of the Haley lab with the demonstrated Pt-RNA biochemistry knowledge of UO colleague Victoria DeRose's lab. The goal of this research is to use chemical biology approaches to identify interactions between platinum-based reagents and cellular constituents. We are developing Pt(II) analogues that are synthetically modified for post-treatment "click"-chemistry, and use these compounds for identifying binding partners and cellular localization. Haley serves as the synthetic advisor/expert for the preparation of the organic ligands and of the complex fluorophores that are subsequently bound/"clicked" to Pt.

Relevant Publications

Multi-functional Pt(II) Reagents: Covalent Modifications of Pt Complexes Enable Diverse Structural Variation and In-cell Detection

White, J. D.; Haley, M. M.; DeRose, V. J. Acc. Chem. Res. 2016, 49, 56-66.
DOI: 10.1021/acs.accounts.5b00322

Azide vs. Alkyne Functionalization in Pt(II) Complexes for Post-Treatment Click Modification: Solid State Structure, Fluorescent Labeling, and Cellular Fate

Wirth, R.; White, J. D.; Moghaddam, A. D.; Ginzburg, A. L.; Zakharov, L. N.; Haley, M. M.; DeRose, V. J. . J. Am. Chem. Soc. 2015, 137, 15169-15175.
DOI: 10.1021/jacs.5b09108

Convenient Detection of Metal-DNA, Metal-RNA, and Metal-Protein Adducts With a Click-Modified Pt(II) Complex

Moghaddam, A. D.; White, J. D.; Cunningham, R. N.; Loes, A. N.; Haley, M. M.; DeRose, V. J. Dalton Trans. 2015, 44, 3536-3539.
DOI: 10.1039/C4DT02649G

An Alkyne-Appended Click-Ready Pt(II) Complex with an Unusual Solid-State Orientation

White, J. D.; Guzman, L. E.; Zakharov, L. N.; Haley, M. M.; DeRose, V. J. Angew. Chem. Int. Ed. 2015, 54, 1032-1035.
DOI: 10.1002/anie.201409853

Platinum-RNA modifications following drug treatment in S. cerevisiae identified by click chemistry and enzymatic mapping

Osborn, M. F.; White, J. D.; Haley, M. M.; DeRose, V. J. ACS Chem. Biol. 2014, 9, 2404-2411.
DOI: 10.1021/cb500395z

Picazoplatin, an Azide-Containing Platinum(II) Derivative for Target Analysis by Click Chemistry

White, J. D.; Osborn, M. F.; Moghaddam, A. D.; Guzman, L. E.; Haley, M. M.; DeRose, V. J. J. Am. Chem. Soc. 2013, 135, 11680-11683.
DOI: 10.1021/ja402453k